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By making this substitution at position three, IGF-1 is inhibited from binding to certain proteins in the body, thus increasing the half-life ( Effects of IGF-1 on Growth Hormone Secretion IGF-1 is a peptide that acts similarly to insulin, and its main function in the body is to stimulate growth by increasing GH secretion from the pituitary gland, as well as regulating cell growth and cell death

This makes degradation undetectable without functional assaysmass spectrometry alone won't catch it

This commonly happens at L4-L5 or L5-S1 levels