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The PCR conditions were: 94 C for 5 min

doi:10.1080/09513590.2018.1540578 Agrawal A, Mahey R, Kachhawa G, Khadgawat R, Vanamail P, Kriplani A

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and then at the point of 24 h, the next application (isoprenaline given twice, at 0 and 24 h) for reinfarction induction (assessment at the end of the subsequent 24 h period)