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Next, the supernatant was collected after centrifuging at 1000 rpm for 10 min at 4 C, and then measured at 532 nm with a microplate reader

The skilled artisan could, using procedures well-known in the art, obtain the polynucleotide sequence corresponding to full-length genes (including, but not limited to the full-length coding region), allelic variants, splice variants, orthologs, and/or species homologues of genes corresponding to SEQ ID NO:1, or a deposited clone, relying on the sequence from the sequences disclosed herein or the clones deposited with the ATCC

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Evidence derived from human observational studies, autopsy findings, and experimental models suggests that sustained androgen receptor overstimulation may induce structural, functional, and metabolic alterations that compromise cardiovascular homeostasis ( Cardiovascular pathology represents one of the most clinically relevant consequences of AAS misuse

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