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A total number of 2635 raw movie stacks made of 50 frames each were collected with SerialEM 87 from a single individual session with a GLACIOS 200 kV FEG Cryo-TEM (ThermoFisher Scientific) using a K2 Summit detector (Gatan Inc., USA) at 36000 magnification and pixel size of 1.145 /pixel without pre-exposure and using a defocus of 2.6 to 1.8 m and 50 e - / 2 total dose per stack (1 e - / 2 per frame)

& Sarkar, R

However, another study using rats showed that protein levels of NAMPT, COX1, and PGC-1a were unchanged in skeletal muscles after 4 weeks of treadmill exercise, and NAD + levels or other mitochondrial readouts were not included 308

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