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Results Responses from 1297 participants indicated 508 (39.2%) self-medicated via injection

2.9 Proteomic and mass spectrometry analysis Mtb supernatants were prepared as above described, concentrated by ultrafiltration then denatured and electrophoresed by 4%15% polyacrylamide gel (SDS-PAGE) and stained with Coomassie, according to published protocols with few modifications (Verdoliva et al., 2013)