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Often times, the impurities are highly similar to the desired product, making chromatographic separation difficult and likely time consuming

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The Trojan Horse hypothesis posits that the nanoparticle form facilitates uptake, subsequently dissolving within the acidic confines of organelles like lysosomes to deliver a highly concentrated dose of toxic ions.136,151 The pH-dependent dissolution of ZnO is a classic example:ZnO(s) + 2H+(aq) Zn2+(aq) + H2O(l)While this mechanism is compelling and supported by significant evidence,152 a critical perspective acknowledges the immense difficulty in experimentally deconvoluting these two toxicity vectors

10.1016/j.jconrel.2021.03.033 48 LuY.ZhaoM.PengY.HeS.ZhuX.HuC.et al (2022)

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Zheng ZM, Baker CC (2006) Papillomavirus genome structure, expression, and post-transcriptional regulation