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Histochemical detection of superoxide and hydrogen peroxide In vivo detection of tissue specific ROS (O 2 and H 2 O 2 ) on leaves through infiltration [37]

and subsequent investigators has characterised GHK-Cu as a potent activator of wound-healing cascades, upregulating transforming growth factor- (TGF-) and suppressing inflammatory mediators such as TNF- and IL-6 in pre-clinical models

Blood Cancer J 4:e253, 2014

Barreto R, Albuquerque-Junior RL, Pereira-Filho RN, Lima P, Nunes R, Barreto A, Junior WL, Camargo EA, Quintans-Junior LJ

Analysis of breast tumor tissues confirmed the increased level of DNA damage, most likely attributed to the formation of 4-hydroxy estradiol (4-OHE) mediated by cytochrome P450, which in turn can trigger the formation of ROS such as H 2 O 2 that are catalytically decomposed to OH radicals via the iron-Fenton reaction