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Briefly, 25-30 million cells were collected and washed twice with cold saline (0.9% NaCl), scraped into 1 ml of cold KPBS, and pelleted via centrifugation at 1,000g for 1.5min at 4C

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The lack of a hook effect in degradation assays was particularly apparent in the presence of CRBN overexpression, where ( S )-dHTC1 induces maximal degradation at concentrations spanning more than 3 orders of magnitude (Extended Data Fig

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