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Antibody spots on the arrays were considered positive if they were visible in duplicate

Fish were anesthetized using benzocaine, and the standard length and weight were measured immediately

Notably, using a cut-off for mating that took place more than 150 days following treatment (i.e

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[DOI] [PMC free article] [PubMed] [Google Scholar] 343.Igwe E.O., Roodenrys S., Probst Y.C., do Rosario V., Netzel M.E., Hong H.T., et al

Sections were permeabilized by incubating in two exchanges of PBS containing 0.4% Triton X-100 each for 10 min