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The cells were seeded in 96-well plates at a density of 1 10 4 cells per well and incubated for 24 h to allow for proper attachment and cell adherence

doi: 10.1016/S0001-7310(06)73466-4

Because many age-related defects in multiple tissues are linked to OxS [4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19], the reversal of OxS in the heart, liver and kidneys of old mice in our study suggests that GlyNAC supplementation could play an important role in promoting health in aging, and thereby contributes to increasing the length of life

This clearly shows that SAM supplementation negatively disrupts systemic methyl metabolism

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doi: 10.1016/0076-6879(87)44177-3 42 KriegTHeinRHatamochiAAumailleyM