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Fusion primers with dual-indexed PCR adapters were used to generate the construct library, and short fragments were removed with AMPure beads

Return the vial to cold storage without leaving it out while other tasks are finished

AHK-Cu (Ala-His-Lys copper complex) engages copper-dependent signaling pathways, acting as a copper-binding tripeptide that modulates extracellular matrix remodeling enzyme activity