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Once the beads were in place, the micropipette was removed, and Pura-lube ophthalmic ointment was applied to the eye while the mice were placed on a warm water blanket to recover

Protocol approach 1: immune rebalancing (thymosin alpha-1 plus KPV) Goal: Restore regulatory immune function while reducing inflammatory signaling Components: Thymosin alpha-1: 1.6 mg subcutaneously twice per week (promotes Treg expansion, modulates dendritic cells) KPV: 200-500 mcg daily, orally or subcutaneously (inhibits NF-kB, reduces TNF-alpha and IL-6) Rationale: Thymosin alpha-1 works at the cellular level to rebuild the regulatory arm of the immune system, while KPV addresses the inflammatory signaling that drives acute symptoms

The data acquired for the targeted analysis of the discovery cohort was imported into the Skyline software 57

NCOA4 binds to lysosomes, degrades ferritin and releases Fe2+, leading to an abnormal increase in Fe2+ levels (6264)

This makes it less likely to cause systemic side effects like water retention, insulin resistance, or abnormal growth of tissues

In addition, rhein reduced the LPS-induced expression of the inflammatory factors IL-1 and IL-6 and inhibited the expression of inflammation-related molecules such as TLR4, NLRP3, and caspase 1 [162]