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Concentrations were based on previous studies, and cells were incubated at 37C with 5% CO2 for 4 h ( CD4 + T cells were plated at a density of 1 10 6 cells/well in 24-well plates in 1 ml RPMI 1640 complete medium containing fumaric acid (173 M) or dimethyl sulfoxide (DMSO,ST038, BYT, Shanghai, China) as a vehicle control

Of the ancillary writers, Wilms tumor 1-associating protein (WTAP) is the most notable, and it is known to recruit the METTL3-14 complex to nuclear speckles (97, 98) (Figure 5)

Despite this, the consistent directionality of mechanistic effects particularly Nrf2 activation, nitric oxide restoration, and angiogenic normalization suggests a coherent physiological signal worth pursuing further

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