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The final libraries were quantified using a Qubit Fluorometer (Thermo Fisher Scientific) with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific)

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The protocol had been implemented with the default parameters except for Force field (CHARMm) and Ligand partial charge Method (Momey-Rone) 31,32

In line with the previously shown lack of enzyme expression in normal lung tissue (Supplementary Fig