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Membrane permeabilization was achieved using 0.1% Triton X-100 in PBS for 10 min, followed by immunological blocking with 1% BSA in PBS for 60 min

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Longitudinal studies in spontaneously hypertensive stroke-prone have demonstrated that gut dysbiosis precedes the onset of hypertension and is marked by shifts in bacterial community structure and altered tryptophan-kynurenine metabolism, implicating microbial pathways in the early development of hypertension and related neurovascular complications (Shi et al., 2022)