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Key readout endpoints include serum ALT and AST enzyme release (hepatocyte membrane integrity), hepatic malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) as lipid peroxidation markers, H&E-stained hepatic necrosis area scoring (zones IIII centrilobular pattern), TUNEL-positive hepatocyte apoptosis count, and MPO activity as a proxy for neutrophil infiltration

GhFSN1 positively regulates the formation of cotton fiber SCWs by regulating the expression of its downstream genes GhIRX12 , GhMYB1 , GhGUT1 , and GhDUF231L1